Flavonoid Fraction of Argyreia Nervosa Leaves with Antiulcer Potential in Different Experimental Rat Models

Argyreia nervosa has been used as an antiulcer in ethano medicine. So the aim of the present study was to evaluate the leaves of the plant for its antiulcer potential and to isolate the bioactive compounds. On the basis of the result of the preliminary screening for antiulcer potential of different fractions of methanolic extract of Argyreia nervosa leaves; n-butanol fraction was charged into silica gel column. The two major subfractions (A and B) obtained were screened for their antiulcerogenic activity by using indomethacin induced gastric ulcer model. Subfraction B (4.59 mg/kg, p.o.) has shown more significant antiulcer as well as antioxidant potential than Subfraction A (6.34mg/kg, p.o.). Subfraction B significantly increased superoxide dismutase (SOD), catalase and glutathione (GSH) levels whereas tissue lipid peroxidation and nitrite levels were found to be decreased as compared to the control group. Quercetin and Kaemferol were isolated from the subfraction B. Both are known antiulcer and antioxidant. Hence, the antiulcer potential of n butanol fraction of methanolic extract of A. nervosa may be due to the synergestic effect of Quercetin and Kaemferol.